This research studies the effect of supplemented growth regulators on Phalaenopsis amabilis micropropagation using temporary immersion system (TIS). The experiment results show that MS supplemented with BA 2 mg/l, NAA 1 mg/l, DCC 30 g/l, peptone 1 g/l, sucrose 30 g/l, CW 20%, CA 1 g/l is the most appropriate culture medium for PLBs. MS½ supplemented with BA 2 mg/l, DCC 30 g/l, Peptones 1 g/l, sucrose 30 g/l, CW 20%, CA 0.5 g/l is the best culture medium for shoot regeneration. MS½ supplemented with IBA 2 mg/l, DCC 30 g/l, Peptones 1 g/l, sucrose 30 g/l, CW 20%, CA 1 g/l is the best culture medium for developing roots. The rates of PLBs regeneration and shoot multiplication on TIS are 2-3 times higher than those on agar culture medium.