Storage by controlling the composition of air or oxygen and carbon dioxide concentration settings is called controlled atmosphere storage. Controlled atmosphere storage aims at controlling metabolism of fresh product to extended the shelf-life. The concentration of oxygen and carbon dioxide in product aremaintained at a desired concentration. The objective of this research was to design a control and monitoring system of oxygen, carbon dioxide, temperature and relative humidity in the storage by using Arduino Uno microcontroller. Design approach consists of functional and structural design. The main function of thesystem control were to control gas injection, open and close the solenoid valve, control the amount of injection, and reading the sensor. Measurements and testing included sensor calibration and functional testing of the control and monitoring system with no-load conditions. Analysis of the experiment resultswas conducted by using statistical analysis of repeated measures ANOVA. The result of observation and measurements of temperature with no-load conditions was 9.88ºC, RH 73.73%, concentration of carbon dioxide 2.22% and concentration of oxygen 4.63%. Accuracy between setpoint and actual temperature was of 98.84%. Accuracy between setpoint and actual RH was of 97.55%. Accuracy between setpoint and actual oxygen was of 83.98%. Accuracy between setpoint and actual carbon dioxide was of 74.26%.