Recent researches indicate that traditional plant may act as a wound healing agent, one of them is Karamunting leaves. The aim of this research is to find out the effect of 70% ethanol extract ointment of karamunting leaves on re-epithelialization of incision wound healing on wistar rat’s skin. This research was an experimental research with post-test only control group design. The research subjects were twenty male wistar rats in which further divided to five groups; positive control group (sanoskin® ointment), negative control group (placebo ointment), experimental group 1 (2,5% karamunting leaves ointment), experimental group 2 (5% karamunting leaves ointment) and experimental group 3 (10% karamunting leaves ointment). All groups underwent 2 cm long incision at the back with 0,2 cm depth, parallel with os.vertebrae, within 5 cm from the ear.On the tenth day, wound tissue were taken from all groups sample and processed into histological blood smears stained with H&E to check the thickness and the width of the slit skin epithelium observed with 10x objective lens magnification. The 70% ethanol extract ointment of leaves Karamunting decreased slit thickness epithelium (p<0,05) and minimized slit width epithelium (p<0.05) compared to negative control. 70% ethanol extract ointment of leaves Karamunting increased wound re-epithelialization of incision wound on wistar rat’s skin with an effective concentration of 5%.