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Microbial Conversion of Cassava Stem (Mannihot Esculenta) Cellulose Into Reducing Sugar by Trichoderma Reesei Pk1j2 Image
Conference paper

Microbial Conversion of Cassava Stem (Mannihot Esculenta) Cellulose Into Reducing Sugar by Trichoderma Reesei Pk1j2

Cassava stems can be utilized as an alternative energy source to produce bioethanol due to itscellulose content, polysaccharide compound can not directly used by yeast. Therefore, cellulose need to behydrolyzed into monomers prior to tranformation into ethanol by Saccharomyces cerevisiae. Hydrolysiscan be performed by cellulase‐producing fungi, such as Trichoderma reesei PK1J2 with ammonium sulphateused as nitrogen source. The effect of ammonium sulphate addition to the growth and reducing sugarproduction rate of Trichoderma reesei from cassava stems was investigated. Hydrolysis was conductedwithout pretreatment. Cassava stem was degraded into small pieces with and without addition ofammonium sulphate. The concentration of (NH4)2SO4 were 0%; 0,5%; 1% and 1,5%. During cellulosefermentation of cassava stem, analysis were carried on cellulose enzyme activity, dry matter loss,glucosamine content, and reducing sugar. Initial spore concentration was 106 spores/g of cassava stem.Addition of ammonium sulphate seem to affect the growth but did not affect of reducing sugar productionof Trichoderma reesei, therefore hydrolisis continued without ammonium sulphate addition. Highestlevels of reducing sugars was obtained after three days of fermentation which was 10,828 g/L.
Total Phenolic Contents and Antioxidant Activities of Ten Soybean Promising Lines Tolerant to Acid Soil Image
Conference paper

Total Phenolic Contents and Antioxidant Activities of Ten Soybean Promising Lines Tolerant to Acid Soil

Marginal land such as acid soil has been recently used to cultivate soybean since soybean cultivated areain Java has shifted to other valuable crops. This expansion aims to fulfill national soybean demand and toreduce soybean import as well as to ensure soybean security. Ten soybean promising lines tolerant to acidsoil have been bred to address this issue. Secondary metabolites in soybean, i.e. phenolic compounds varyin each genotype. These compounds function mainly as chemical defense against insects and pathogens.Also, the phenolic compounds including flavonoids which hypothetically have antioxidant activities havepositive effects to human health. Phenolic contents of ten soybean promising lines ranged from 2.87 to3.73 mg GAE/g. Five varieties, i.e. Wilis, Tanggamus, Anjasmoro, Grobogan, and Argomulyo as the checkshad the phenolic contents of 3.02 to 3.93 mg GAE/g. Flavonoid contents of the ten lines varied from 0.57to 0.76 mg CAE/g. There were no check varieties which had the flavonoid contents higher than those ofthe promising lines. The Tgm/Anj‐784, Tgm/Anj‐856, Tgm/Anj‐844, and Tgm/Anj‐857 had the equallyhigh flavonoid contents, i.e. 0.76, 0.75, 0.72, and 0.72 mg CAE/g, respectively. Antioxidant activities as indicatedby DPPH values of the ten soybean lines were lower than those of Wilis, Argomulyo, andGrobogan.
Potential of Indigenous Probiotic Lactobacillus Plantarum Dad 13 as Anti‐diarrhea and Immuno‐modulator Image
Potential of Indigenous Probiotic Lactobacillus Plantarum Dad 13 as Anti‐diarrhea and Immuno‐modulator Image
Conference paper

Potential of Indigenous Probiotic Lactobacillus Plantarum Dad 13 as Anti‐diarrhea and Immuno‐modulator

Characteristics Of Purse‐cowpea Composite Flour Biscuits On Different Types Of Packaging During Storage Image
Characteristics Of Purse‐cowpea Composite Flour Biscuits On Different Types Of Packaging During Storage Image
Conference paper

Characteristics Of Purse‐cowpea Composite Flour Biscuits On Different Types Of Packaging During Storage

Application of Biothechnology Agricultural by Manure, Effort of Sesame Production in Coastal Sandy Land Image
Application of Biothechnology Agricultural by Manure, Effort of Sesame Production in Coastal Sandy Land Image
Conference paper

Application of Biothechnology Agricultural by Manure, Effort of Sesame Production in Coastal Sandy Land

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Anti‐diabetic Activity of Sambiloto Extract (Andrographis Paniculata Ness) to Decrease Blood Glucose Level of Aloxan‐induced Diabetic Rat Image
Conference paper

Anti‐diabetic Activity of Sambiloto Extract (Andrographis Paniculata Ness) to Decrease Blood Glucose Level of Aloxan‐induced Diabetic Rat

This research aimed to measure dosage of sambiloto (Andrographis paniculata Ness) extract to decreaseblood glucose level of diabetic rats (Rattus norvegicus). Completely randomized design was applied forexperiment in triplicate sampling and 7 treatments: D0, D1, D2 of normal, diabetic, and 0.9 mg glibenclamidecontrol, respectively; D3, D4, D5 of 100, 200, 400 mg sambiloto extract respectively; and D6 of 200mg sambiloto + 0.9 mg glibenclamide; all dosage in 200 g body weight basis of 2 months old male whiteWistar rats weigh ± 200 g for 21 days. Anova and Kruskal‐Wallis were used as statistic analysis; results thenfurtherly analyzed using Moods Median Test at α 5%. Results showed that sambiloto extract at dosage of100 mg, 200 mg, 400 mg/200 g body weight as well as combination of 200 mg sambiloto + 0.9 glibenclamideable to reduce rat blood glucose level. Interestingly, 400 mg sambiloto/200 g body weight was comparableto the result obtain using 0.9 mg glibenclamide/200 g body weight of 56%. While rats treated withcombination of 200 mg sambiloto + 0.9 mg glibenclamide/200 g body weight had 58% lower blood glucosecompare to initial blood glucose level, however hypoglycemic risk need to be considered.
Antimicrobial Test Of “Tutup” Flowers (Macaranga Tanarius (L.) Mull.arg.) Image
Conference paper

Antimicrobial Test Of “Tutup” Flowers (Macaranga Tanarius (L.) Mull.arg.)

Lignin Content in Fermentation of Cocoa Pod Husk (Theobroma Cocoa) Used Phanerochaete Chrysosporium Image
Conference paper

Lignin Content in Fermentation of Cocoa Pod Husk (Theobroma Cocoa) Used Phanerochaete Chrysosporium

Salted Egg Interior Quality With Starfruit Extrac Image
Conference paper

Salted Egg Interior Quality With Starfruit Extrac

The Effect of Acetic Acid on Characteristics of Tuna Fish Skin Gelatin Image
Conference paper

The Effect of Acetic Acid on Characteristics of Tuna Fish Skin Gelatin

Analysis of Customer Preference on the Cowpea Tempe Image
Analysis of Customer Preference on the Cowpea Tempe Image
Conference paper

Analysis of Customer Preference on the Cowpea Tempe

Antimicrobial Test Of “Tutup” Flowers (Macaranga Tanarius (L.) Mull.arg.) Image
Antimicrobial Test Of “Tutup” Flowers (Macaranga Tanarius (L.) Mull.arg.) Image
Conference paper

Antimicrobial Test Of “Tutup” Flowers (Macaranga Tanarius (L.) Mull.arg.)

Salted Egg Interior Quality With Starfruit Extrac Image
Salted Egg Interior Quality With Starfruit Extrac Image
Conference paper

Salted Egg Interior Quality With Starfruit Extrac

The Effect of Acetic Acid on Characteristics of Tuna Fish Skin Gelatin Image
The Effect of Acetic Acid on Characteristics of Tuna Fish Skin Gelatin Image
Conference paper

The Effect of Acetic Acid on Characteristics of Tuna Fish Skin Gelatin

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Screening of Soybean Germplasm Collection Resistance to Rust Disease (Phakopsora Pachyrhizi) Image
Conference paper

Screening of Soybean Germplasm Collection Resistance to Rust Disease (Phakopsora Pachyrhizi)

Rust disease is an important disease on soybean, it was widely distributed in almost all soybeanproducing countries, the yield losses can be reach 85 %. One of the control measured is planting theresistant varieties. Resistant gens of one character can be obtained from the germplasm collection. Thestudy aiar to evaluate the resistance of soybean germplasm collection against rust diseases. The study wasconducted at Kendalpayak experimental station, Indonesian Legumes and Tuber Crops ResearchInstitute, in June to September 2013. Materials used were 175 genotypes of soybean germplasm collection.Observations of rust disease resistance based on the method of International Working Group on SoybeanRust system. The results showed that out of 175 soybean genotypes tested, 162 genotypes were moderatelyresistant, 12 genotypes were moderately susceptible , and one genotype was susceptible to rust disease.