The use of combinations of microorganisms as feed additives can synergize to produce enzymes to help the digestive process of feed. This study aims to determine the best combination of microorganisms as probiotics in feeding on growth performance, gastric evacuation, and blood glucose levels of milkfish. Milkfish with an initial weight of 9.21±0.14 g, reared at a density of 20 fish in an aquarium measuring 50 x 40 x 35 cm3 containing 48 L of water with 20 ppt salinity and equipped with a recirculation system. The research design was Completely Randomized Design with five treatments, namely: control (feed without microorganisms), Bacillus sp. and Lactobacillus sp., Rhizophus sp. and Aspergillus sp., Saccharomyces sp. and Trichoderma sp., and Mixed microorganisms, Bacillus sp., Lactobacillus sp., Rhizophus sp., Aspergillus sp., Saccharomyces sp., and Trichoderma sp. During 50 days of rearing, the fish were given experimental feed three times a day at 07.00, 12.00, and 15.00 at 5% body weight. The results showed that the treatment significantly affected growth, feed efficiency, and hepatosomatic index. However, it did not affect milkfish's survival and liver and muscle glycogen levels. Absolute growth (30.79±0.87 g), relative growth rate (333.91±5.83%), biomass growth (615.76±17.48%), feed efficiency (57.26±0.39 %), and the best hepatosomatic index (0.99±0.01) were obtained in the mixed microorganism treatment, and mg/g, and muscle 5.45-5.72 mg/g. The gastric evacuation rate and the Fastest peak and peak decrease in blood glucose levels were obtained in the mixed microorganisms treatment at 5 and 3-4 hours postprandial. The diverse microorganisms can be added to the feed at a dose of 10 mL/kg to support the intensification of milkfish culture