With the increasing mobility of people, or items that can be a carrier of pests and diseases in animals and plants, and limited oversight, security and law enforcement, then the chances of the spread of pests and diseases is quite large. It would be very harmful to the preservation of natural resources and the national economy. Efforts to prevent the entry into the territory of the Republic of Indonesia of pests and diseases that have the potential damage done through quarantine. In Act 16 of 1992 concerning Animal, Fish and Plant Quarantine is a retreat from or acts as an effort to prevent the entry and spread of pests and diseases from abroad and from one area to another within the country or the release of the the territory of the Republic of Indonesia. Law enforcement Article 31 of Law 16 of 1992 at the Port of Pontianak Dwikora which can not be implemented as it should be this is due to several factors. Where this study using sociological juridical approach to the specification of descriptive analysis and analyzed using qualitative methods. Expected to study law enforcement in dealing with criminal offenses in the field of quarantine of animals, fish and plants can be effective.