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Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures Image
Journal article

Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures

Identification of the Causal Agent of Cocoa Pod Rot Disease From Various Locations Image
Identification of the Causal Agent of Cocoa Pod Rot Disease From Various Locations Image

Identification of the Causal Agent of Cocoa Pod Rot Disease From Various Locations

The Role of Turnera Subulata and Cosmos Sulphureus Flowers in the Life of Anagrus Nilaparvatae (Hymenoptera: Mymaridae) Image
The Role of Turnera Subulata and Cosmos Sulphureus Flowers in the Life of Anagrus Nilaparvatae (Hymenoptera: Mymaridae) Image

The Role of Turnera Subulata and Cosmos Sulphureus Flowers in the Life of Anagrus Nilaparvatae (Hymenoptera: Mymaridae)

Influence of Culture Medium on the Sporulation and Viability of Aspergillus Spp. and Talaromyces Spp. Entomopathogenic Fungi Image
Influence of Culture Medium on the Sporulation and Viability of Aspergillus Spp. and Talaromyces Spp. Entomopathogenic Fungi Image

Influence of Culture Medium on the Sporulation and Viability of Aspergillus Spp. and Talaromyces Spp. Entomopathogenic Fungi

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
Journal article

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia

Diversity of Feed Storage Pest Beetle in Banten Province Image
Journal article

Diversity of Feed Storage Pest Beetle in Banten Province

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
Journal article

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia

Diversity of Feed Storage Pest Beetle in Banten Province Image
Diversity of Feed Storage Pest Beetle in Banten Province Image
Journal article

Diversity of Feed Storage Pest Beetle in Banten Province

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Optimasi Metode PCR untuk Deteksi Pectobacterium Carotovorum, Penyebab Penyakit Busuk Lunak Anggrek Image
Journal article

Optimasi Metode PCR untuk Deteksi Pectobacterium Carotovorum, Penyebab Penyakit Busuk Lunak Anggrek

Soft rot is one of the most important diseases of orchids caused by Pectobacterium carotovorum. The conventional methods for the detection of pathogen is tedious and time consuming. In recent years, numerous molecular diagnostic approaches for the detection of P. carotovorum have been developed, including various PCR-based assays. Optimization of PCR technique to DNA amplification is essential for time and material efficiency, which will make detection to be rapid and more appropriate. The purposes of this study were to decide concentration of DNA and primer, and also the concentration of bacterial pure cultures and primer to amplify 16S rRNA gene fragment. Optimization of PCR was done by using various concentration of DNA, pure cultures of bacteria, and primer to amplify the 16S rRNA gene sequence. The results showed that the most optimum concentration to amplify 16S rRNA gene sequence at DNA and primer concentration were 63,4 ng/µl and 10 pmol, while pure cultures and primer concentrations were at 8×109 CF U/ml and 10 pmol respectively. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum merupakan salah satu penyakit penting pada tanaman anggrek. Deteksi patogen secara cepat dan akurat dapat dilakukan secara molekular menggunakan teknik Polymerase chain reaction (PCR). Optimasi metode PCR perlu dilakukan untuk mengefisienkan waktu dan penggunaan bahan sehingga proses deteksi dapat dilakukan dengan cepat dan tepat. Penelitian ini bertujuan untuk menentukan konsentrasi DNA dengan primer maupun konsentrasi kultur murni bakteri dengan primer yang paling tepat untuk mendapatkan fragmen gen 16S rRNA. Optimasi PCR dilakukan menggunakan beberapa variasi pengenceran pada DNA, kultur murni bakteri, dan primer untuk mengamplifikasi gen 16S rRNA. Hasil penelitian menunjukkan bahwa konsentrasi yang paling optimal untuk mengamplifikasi gen 16S rRNA yaitu DNA dan primer masing-masing sebesar 63,4 ng/µl dan 10 pmol, sedangkan konsentrasi kultur murni dan primer sebesar 8×109 CFU/ml dan 10 pmol.
Identifikasi Morfologi Beberapa Spesies Jamur Fusarium Image
Journal article

Identifikasi Morfologi Beberapa Spesies Jamur Fusarium

The research was conducted to study the morphological difference based on macroscopic and microscopic appearance of several Fusarium spp. Fusarium spp. isolates were propagated onto Potato Dextrose Agar (PDA) by using single-spore method. All isolates were observed macroscopically and microscopically to determine colony appearance, colony growth diameter and formation of macroconidia, microconidia and conidiophores. The results showed that colony appearance of all isolates was similar. Therefore they could not clearly be differentiated by one to another. On the other hand, microscopic observation showed that there was different conidiophore morphology of F. oxysporum and other Fusarium spp. Microscopic morphology among F. oxysporum isolates were difficult to differentiate. Microconidia were produced in false-head which was the characteristic feature of most F. oxysporum. In conclusion, microscopic morphology observation could only be able to differentiate Fusarium spp. isolates at species level, but not to formae speciales. Penelitian ini bertujuan untuk mengetahui perbedaan morfologi berdasarkan morfologi makroskopis dan mikroskopis beberapa isolat Fusarium spp. Isolat Fusarium spp. diperbanyak pada medium Potato Dextrose Agar (PDA) dengan menggunakan teknik monospora. Semua isolat ini kemudian diamati secara makroskopis dan mikroskopis untuk mengetahui morfologi koloni, pertumbuhan koloni, bentuk makrokonidium dan mikrokonidium serta konidiofornya. Hasil penelitian menunjukkan bahwa sebagian besar isolat mempunyai morfologi koloni yang hampir sama, sehingga tidak bisa dibedakan dengan jelas satu dengan lainnya. Akan tetapi, pengamatan secara mikroskopis menunjukkan adanya perbedaan morfologi konidiofor pada isolat F. oxysporum dengan Fusarium spp., sementara morfologi mikroskopis antara isolat Fusarium spp. sulit untuk dibedakan. Mikrokonidium yang dibentuk pada false-head merupakan karakter utama F. oxysporum. Dari hasil penelitian ini dapat disimpulkan bahwa morfologi mikroskopis hanya dapat digunakan untuk membedakan Fusarium spp pada tingkat spesies, tetapi tidak pada tingkat forma spesialis.
Ketahanan Jamur terhadap Fungisida di Indonesia Image
Journal article

Ketahanan Jamur terhadap Fungisida di Indonesia

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Journal article

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun

Biological Control of Plant Disease Caused by Bacteria Image
Journal article

Biological Control of Plant Disease Caused by Bacteria

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Journal article

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon

Ketahanan Jamur terhadap Fungisida di Indonesia Image
Ketahanan Jamur terhadap Fungisida di Indonesia Image
Journal article

Ketahanan Jamur terhadap Fungisida di Indonesia

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Journal article

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Journal article

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon

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Beberapa Catatan Mengenai Hama Baru: Penggulung Daun Teh Siput tanpa Cangkang, Parmarion Pupillaris Image
Journal article

Beberapa Catatan Mengenai Hama Baru: Penggulung Daun Teh Siput tanpa Cangkang, Parmarion Pupillaris

In the past tea plantation at Pagilaran estate is attacked by leaf roller pests. These are Homona coffearia, Cydia leucostoma, and Gracilaria theifora. At present a slug Parmarion pupillaris is found and considered as a new leaf roller pest, because they live inside of the rolled leaf. This symptom is not known before. The population of Parmarion slug is high 3-4 slugs per bush of tea at the sub-division of Pagilaran estate of Pagilaran, Binorong and Garjito. This condition maybe supported by the weather that Pagilaran estate have a high rainfall of 4,842-6,067 mm per year with only 1-2 months of dry season.
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