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Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
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Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia

Diversity of Feed Storage Pest Beetle in Banten Province Image
Journal article

Diversity of Feed Storage Pest Beetle in Banten Province

Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures Image
Journal article

Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia Image
Journal article

Respons Awal Ketahanan Jagung terhadap Peronosclerospora Maydis dan Induksi Bahan Kimia

Diversity of Feed Storage Pest Beetle in Banten Province Image
Diversity of Feed Storage Pest Beetle in Banten Province Image
Journal article

Diversity of Feed Storage Pest Beetle in Banten Province

Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures Image
Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures Image
Journal article

Characteristics and Toxicity of Nanoemulsion Formulation of Piper Retrofractum and Tagetes Erecta Extract Mixtures

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Optimasi Metode PCR untuk Deteksi Pectobacterium Carotovorum, Penyebab Penyakit Busuk Lunak Anggrek Image
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Optimasi Metode PCR untuk Deteksi Pectobacterium Carotovorum, Penyebab Penyakit Busuk Lunak Anggrek

Soft rot is one of the most important diseases of orchids caused by Pectobacterium carotovorum. The conventional methods for the detection of pathogen is tedious and time consuming. In recent years, numerous molecular diagnostic approaches for the detection of P. carotovorum have been developed, including various PCR-based assays. Optimization of PCR technique to DNA amplification is essential for time and material efficiency, which will make detection to be rapid and more appropriate. The purposes of this study were to decide concentration of DNA and primer, and also the concentration of bacterial pure cultures and primer to amplify 16S rRNA gene fragment. Optimization of PCR was done by using various concentration of DNA, pure cultures of bacteria, and primer to amplify the 16S rRNA gene sequence. The results showed that the most optimum concentration to amplify 16S rRNA gene sequence at DNA and primer concentration were 63,4 ng/µl and 10 pmol, while pure cultures and primer concentrations were at 8×109 CF U/ml and 10 pmol respectively. Penyakit busuk lunak yang disebabkan oleh Pectobacterium carotovorum merupakan salah satu penyakit penting pada tanaman anggrek. Deteksi patogen secara cepat dan akurat dapat dilakukan secara molekular menggunakan teknik Polymerase chain reaction (PCR). Optimasi metode PCR perlu dilakukan untuk mengefisienkan waktu dan penggunaan bahan sehingga proses deteksi dapat dilakukan dengan cepat dan tepat. Penelitian ini bertujuan untuk menentukan konsentrasi DNA dengan primer maupun konsentrasi kultur murni bakteri dengan primer yang paling tepat untuk mendapatkan fragmen gen 16S rRNA. Optimasi PCR dilakukan menggunakan beberapa variasi pengenceran pada DNA, kultur murni bakteri, dan primer untuk mengamplifikasi gen 16S rRNA. Hasil penelitian menunjukkan bahwa konsentrasi yang paling optimal untuk mengamplifikasi gen 16S rRNA yaitu DNA dan primer masing-masing sebesar 63,4 ng/µl dan 10 pmol, sedangkan konsentrasi kultur murni dan primer sebesar 8×109 CFU/ml dan 10 pmol.
Identifikasi Morfologi Beberapa Spesies Jamur Fusarium Image
Journal article

Identifikasi Morfologi Beberapa Spesies Jamur Fusarium

The research was conducted to study the morphological difference based on macroscopic and microscopic appearance of several Fusarium spp. Fusarium spp. isolates were propagated onto Potato Dextrose Agar (PDA) by using single-spore method. All isolates were observed macroscopically and microscopically to determine colony appearance, colony growth diameter and formation of macroconidia, microconidia and conidiophores. The results showed that colony appearance of all isolates was similar. Therefore they could not clearly be differentiated by one to another. On the other hand, microscopic observation showed that there was different conidiophore morphology of F. oxysporum and other Fusarium spp. Microscopic morphology among F. oxysporum isolates were difficult to differentiate. Microconidia were produced in false-head which was the characteristic feature of most F. oxysporum. In conclusion, microscopic morphology observation could only be able to differentiate Fusarium spp. isolates at species level, but not to formae speciales. Penelitian ini bertujuan untuk mengetahui perbedaan morfologi berdasarkan morfologi makroskopis dan mikroskopis beberapa isolat Fusarium spp. Isolat Fusarium spp. diperbanyak pada medium Potato Dextrose Agar (PDA) dengan menggunakan teknik monospora. Semua isolat ini kemudian diamati secara makroskopis dan mikroskopis untuk mengetahui morfologi koloni, pertumbuhan koloni, bentuk makrokonidium dan mikrokonidium serta konidiofornya. Hasil penelitian menunjukkan bahwa sebagian besar isolat mempunyai morfologi koloni yang hampir sama, sehingga tidak bisa dibedakan dengan jelas satu dengan lainnya. Akan tetapi, pengamatan secara mikroskopis menunjukkan adanya perbedaan morfologi konidiofor pada isolat F. oxysporum dengan Fusarium spp., sementara morfologi mikroskopis antara isolat Fusarium spp. sulit untuk dibedakan. Mikrokonidium yang dibentuk pada false-head merupakan karakter utama F. oxysporum. Dari hasil penelitian ini dapat disimpulkan bahwa morfologi mikroskopis hanya dapat digunakan untuk membedakan Fusarium spp pada tingkat spesies, tetapi tidak pada tingkat forma spesialis.
Ketahanan Jamur terhadap Fungisida di Indonesia Image
Journal article

Ketahanan Jamur terhadap Fungisida di Indonesia

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Journal article

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun

Biological Control of Plant Disease Caused by Bacteria Image
Journal article

Biological Control of Plant Disease Caused by Bacteria

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Journal article

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon

Ketahanan Jamur terhadap Fungisida di Indonesia Image
Ketahanan Jamur terhadap Fungisida di Indonesia Image
Journal article

Ketahanan Jamur terhadap Fungisida di Indonesia

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun Image
Journal article

Identifikasi Penyebab Penyakit Daun Keriting Kuning Pada Tanaman Mentimun

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon Image
Journal article

Identifikasi Molekuler Virus Penyebab Penyakit Daun Keriting Isolat Bantul pada Melon

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Pengaruh Waktu Infeksi Virus Kerdil Pisang Terhadap Kerentanan Tiga Kultivar Image
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Pengaruh Waktu Infeksi Virus Kerdil Pisang Terhadap Kerentanan Tiga Kultivar

Effects of time of infection of banana bunchy top virus on susceptibility of three banana cultivars. Banana Bunchy Top, caused by Banana Bunchy Top Virus (BBTV), is one of the most important banana diseases in Indonesia. Approach to reduce disease incidence involves prevention of early infection especially on susceptible cultivars. This study was conducted to evaluate the response of three banana cultivars, Ambon Kuning, Tanduk, and Kepok, to different time of infection of BBTV i.e., one week and three week after adaptation period, and one week during adaptation period. Banana plants used in the study were prepared through in vitro propagation (tissue culture) and virus transmission was done using aphid vector, Pentalonia nigronervosa. In addition to observation on symptom expression, inhibition of plant height, and reduction of leaf size, conformation of virus infection was done through indirect ELISA. Virus concentration on different part of the plant, young leaf, stem, and root, tends to decrease over the time due to the ability of BBTV to move from cell to cell before replication takes place. It is evidenced that BBTV was able to infect banana in all growth stages although the younger plant is more susceptible to BBTV. Although concentration of the virus in the tested plant is considered high, symptoms expression of BBTV infection can be differentiated from moderate to very severe. Response of banana plants to infection of BBTV can be grouped into susceptible (Ambon Kuning), moderate tolerant (Tanduk), and tolerant (Kepok).
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