Bacillus thuringiensis var.Israelensis (Bti) is bacterium which is potential as bioinsecticidies. However, it is difficult for multiply bacterium on a large scale due to difficulty in obtaining standard media to develop Bti. This study aimed to find out whether Bti can grow in Mekongga rice dishwater media and effective to eliminate Ae.aegypti larvae strain Kendari, to find out the larvicide power, to find out the value of LT50, and to find out the residual effect. Method of this study was laboratory experimental with post test only one group control design. Twenty five Ae.aegypti instar III larvae was used in this research. The result showed Bti can grow in Mekongga rice dishwater media and effective in eliminating the Ae.aegypti larvae strain Kendari after 24 hours exposure, with LC50 and LC90 are 3,321 ppm and 4,945 pp respectively, while in 48 hours exposure with LC50 and LC90 are 3,119 ppm and 4,945 ppm respectively. The values of LT50 Bti on Mekongga rice dishwater media is 45,49 hours and the residual effect is 4 days. Anova analysis show significant differences on various ceoncentrations of Bti in Mkongga rice dishwater media againts the mortality of Ae.aegypti larvae strain Kendari.The study proves that the Mekongga rice dishwater is a potential media which can be alternative media to grow Bti which is pathogen against Ae.aegypti starin kendari.